How to Calculate Absorption Using Beer-Lambert Law

How can we calculate the absorption of DTC at λmax using the Beer-Lambert law?

Calculation of Absorption Using Beer-Lambert Law

The Beer-Lambert law, also known as the Beer's law, is used to relate the absorption of light to the properties of the material absorbing the light. It is commonly used in chemistry to determine the concentration of a substance in a solution based on its absorbance.

The absorption of DTC at λmax can be calculated using the Beer-Lambert law equation as: A = εcl

Where:

  • A is the absorbance
  • ε is the molar absorptivity (specific to the substance and wavelength)
  • c is the concentration of the solution in moles per liter (M)
  • l is the path length that the light travels through the solution in centimeters (cm)

Therefore, to calculate the absorption of DTC at λmax, you would use the formula:

A = (8.5 x 10^4 L/(mol·cm)) x (1.5 x 10^-5 M) x l

The resulting value of A will depend on the specific path length (l) used in the calculation.

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