Colorimeter Calibration for Green Dye: Let's Dive into Absorbance and Concentration Calculation!
a. What is the absorbance of the 2.00 ppt, 6.00 ppt, and 10.00 ppt solutions of green dye?
1. 2.00 ppt solution transmits 85.7% of the light.
2. 6.00 ppt solution transmits 63.0% of the light.
3. 10.00 ppt solution transmits 46.3% of the light.
Absorbance Calculation:
1. Absorbance of 2.00 ppt solution: A = -log(85.7/100) = -log(0.857) ≈ 0.067.
2. Absorbance of 6.00 ppt solution: A = -log(63.0/100) = -log(0.630) ≈ 0.200.
3. Absorbance of 10.00 ppt solution: A = -log(46.3/100) = -log(0.463) ≈ 0.333.
In colorimeter calibration, the absorbance of a solution is calculated using the formula A = -log(%T/100), where A is the absorbance and %T is the percentage of light transmitted through the solution. By applying this formula to the given data, we can determine the absorbance values for the 2.00 ppt, 6.00 ppt, and 10.00 ppt solutions of green dye.
The absorbance values are approximately 0.067, 0.200, and 0.333 for the 2.00 ppt, 6.00 ppt, and 10.00 ppt solutions, respectively. These values indicate the extent to which each solution absorbs light, with higher absorbance values corresponding to lower percentages of transmitted light.