Identifying and Introducing Plasmid Fusion into Cells
What is the process of identifying and introducing a plasmid fusion into cells?
How can the correct plasmid be identified from the E. coli library?
Identifying and Introducing Plasmid Fusion
The process of identifying and introducing a plasmid fusion into cells involves several steps. You are correct that the first step is to identify the correct plasmid from the E. coli library. You can do this by sequencing each plasmid and identifying the gene of interest using NCBI or IMG databases. Once the E. coli strain containing the desired plasmid is identified, it can be recovered from the master plate using the numbering and lettering system that is in place. To locate the E. coli cells that contain the functional genomics plasmid for TagCFP or mCherry translation fusion, look for the corresponding identifier on the master plate. Afterwards, this strain can be mobilized into the target strain in order to insert the plasmid fusion. Please remember that this process should be performed carefully in a suitable environment following laboratory safety guidelines.
To introduce a plasmid fusion into cells, the correct plasmid would have to be identified in the E. coli library and then mobilised from that strain into the target strain. The E. coli library strain containing the correct plasmid could be identified from the master plate below.
The E. coli strain that contains the desired plasmid for TagCFP or mCherry translation fusion can be identified by sequencing each plasmid and using databases like NCBI or IMG to identify the gene of interest.
The cells with the identified plasmid can then be recovered from the master plate using its numbering and lettering system, and the plasmid fusion can be introduced into the target strain.
The process of identifying and introducing a plasmid fusion into cells involves several steps. You are correct that the first step is to identify the correct plasmid from the E. coli library. You can do this by sequencing each plasmid and identifying the gene of interest using NCBI or IMG databases. Once the E. coli strain containing the desired plasmid is identified, it can be recovered from the master plate using the numbering and lettering system that is in place. To locate the E. coli cells that contain the functional genomics plasmid for TagCFP or mCherry translation fusion, look for the corresponding identifier on the master plate. Afterwards, this strain can be mobilized into the target strain in order to insert the plasmid fusion. Please remember that this process should be performed carefully in a suitable environment following laboratory safety guidelines.