Autoclave Sterilization: What Materials Can Be Sterilized?
Which materials can be sterilized in an autoclave?
Choose the correct options:
- a. Steel shaft and impeller of a bioreactor
- b. Glass petri dishes
- c. Glass bioreactor
- d. Micronutrient solution containing metals
- e. Plastic bioreactor
- f. Plastic pipettes
- g. Media containing proteins
Final answer:
An autoclave can sterilize the steel shaft and impeller of a bioreactor (Option A), glass petri dishes (Option B), and a glass bioreactor (Option C).
Answer:
An autoclave can sterilize the steel shaft and impeller of a bioreactor, glass petri dishes, and a glass bioreactor.
Autoclave sterilization is a critical process in many laboratory settings to ensure the complete elimination of harmful microorganisms. An autoclave is a device that uses high pressure and steam to kill bacteria, viruses, fungi, and spores on items placed inside it.
The materials that can safely undergo autoclave sterilization include the steel shaft and impeller of a bioreactor, glass petri dishes, and glass bioreactors. These items can withstand the high temperature and pressure inside the autoclave without being damaged.
On the other hand, plastic items such as plastic bioreactors and plastic pipettes cannot be sterilized in an autoclave. The high heat and pressure of the autoclave can cause plastic materials to warp or melt, rendering them useless for future use.
Solutions containing proteins or nutrients are also unsuitable for autoclave sterilization. The heat from the autoclave can denature proteins and alter the chemical composition of the solution, making it ineffective for its intended purpose.
Therefore, it is essential to carefully choose the materials that can be sterilized in an autoclave to ensure the safety and efficacy of laboratory procedures.